ZearalaTest WB

Zearalenone is an estrogenic mycotoxin produced by the fungus Fusarium graminearum. Zearalenone induces feminization in animals at concentrations in feed of about 1 mg/kg. Higher concentrations (50–100 mg/kg) can interfere with conception, ovulation, implantation, fetal development, and the viability of newborn animals. Increasingly concerned with human ingestion of this mycotoxin, the European Union is considering a full slate of proposed regulations for zearalenone in foods ranging from bread to breakfast cereals.

Using monoclonal antibody-based affinity chromatography, ZearalaTest™WB is a new HPLC-only test for the detection of zearalenone using wide bore immunoaffinity columns.With a total volume of 3 mL, compared to the total of 1 mL in VICAM’s standard columns, ZearalaTest WB allows for a faster flow rate preferred by many laboratories and is the ideal cleanup step for any HPLC.

ZearalaTest WB is one of many wide bore columns available from VICAM. Through the development of this fast-flow WB product line,VICAM demonstrates a commitment to improving mycotoxin testing and responding to our customers’needs.

  • ZearalaTest WB Datasheet ZearalaTest WB Datasheet (533 KB)
  • Instruction Manual
  • Ordering Info
  • Cat. No.
    Description
    G1026
    ZearalaTestWB Columns (25 per Box)
    31240 
    VICAM Fluted Filter Paper, 24 cm (100)
    31955 
    Microfiber Filters, 1.5 μm, 11 cm (100)
    34000 
    Disposable Cuvettes (250 per Pack)
    35016 
    Methanol, HPLC Grade (4 X 4 L)
    G1124 
    Noniodized Sodium Chloride (Salt,NaCl)
    G1118 
    WB Column Coupling (6)
    20050 
    Graduated Cylinder, 50 mL
    20100 
    Digital Scale with AC Adapter
    20200 
    Commercial Blender with Stainless Steel Container
    20300 
    Glass Blender Jar
    20250 
    Graduated Cylinder, 250 mL
    20700 
    Wash Bottle, 500 mL
    21010 
    Cuvette Rack
    21020 
    Single Position Pump Stand†
    23040 
    Vortex Mixer
    36020 
    Filter Funnel, 65 mm (10 per Pack)
    36022 
    Filter Funnel, 105 mm (4 per Pack)
    20656 
    Micro-pipette Tips for 1 mL Micro-pipettor (100)
    G4033 
    Micro-pipettor, 1 mL

    Also required; must be sourced locally: Distilled, Reverse Osmosis, or Deionized Water
    Zearalenone Standard 50 ng/μL from Supelco

    †Multiple position pump stands available

BENEFITS

  • Exclusive – Specifically for HPLC use
  • Durable – Long shelf life; requires no refrigeration
  • Versatile – For use with a variety of samples
  • Quick –20 minutes to isolate toxin*
  • Wide Range – Detects levels as high as 1500 ng
  • Fast Flow – Passes more volume over the column
  • *excluding preparation and extraction

*excludes preparation and extraction ** 1 ppb for grain and nuts