Aflatoxins are carcinogenic compounds produced by fungi, and are controlled worldwide via regulation, including maximum levels in food and feed. This application note describes the improvement of an existing AOAC method using the Waters Arc HPLC System with fluorescence detection. Effective sample clean-up step was achieved using AflaTest™ WB immunoaffinity chromatography (IAC). Recoveries in corn or peanuts were greater than 90%, thus meeting the EU criteria. The method has been demonstrated to be suitable for checking compliance with regulatory limits.
